Human Angiogenesis Array (23 targets) (SARB0020)

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SKU:
SARB0020
Product Type:
Protein Array
Analytes:
Angiopoietin-1
Analytes:
Angiopoietin-2
Analytes:
Angiostatin
Analytes:
Endostatin
Analytes:
GCSF
Analytes:
GM-CSF
Analytes:
I-309 (TCA-3/CCL1)
Analytes:
IL-10
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-2
Analytes:
IL-4
Analytes:
I-TAC (CXCL11)
Analytes:
MCP-3 (MARC/CCL7)
Analytes:
MCP-4 (CCL13)
Analytes:
MMP-1
Analytes:
MMP-9
Analytes:
PECAM-1 (CD31)
Analytes:
Tie-2
Analytes:
TNF alpha
Analytes:
uPAR
Analytes:
VEGFR2
Analytes:
VEGFR3
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0020
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:23
Gene Symbols:ANGPT1, ANGPT2, CCL1, CCL13, CCL7, COL18A1, CSF2, CSF3, CXCL11, FLT4|IL10, IL1A, IL1B, IL2, IL4, KDR, MMP1, MMP9, PECAM1, PLAUR, PLG, TEK, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Angiopoietin-1
  • Gene Symbol: ANGPT1
  • Gene ID: 284
  • Accession #: Q15389
Angiopoietin-2
  • Gene Symbol: ANGPT2
  • Gene ID: 285
  • Accession #: O15123
Angiostatin
  • Gene Symbol: PLG
  • Gene ID: 5340
  • Accession #: P00747
Endostatin
  • Gene Symbol: COL18A1
  • Gene ID: 80781
  • Accession #: P39060
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: 6373
  • Accession #: O14625
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
MCP-4 (CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
MMP-1
  • Gene Symbol: MMP1
  • Gene ID: 4312
  • Accession #: P03956
MMP-9
  • Gene Symbol: MMP9
  • Gene ID: 4318
  • Accession #: P14780
PECAM-1 (CD31)
  • Gene Symbol: PECAM1
  • Gene ID: 5175
  • Accession #: P16284
Tie-2
  • Gene Symbol: TEK
  • Gene ID: 7010
  • Accession #: Q02763
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
uPAR
  • Gene Symbol: PLAUR
  • Gene ID: 5329
  • Accession #: Q03405
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: 3791
  • Accession #: P35968
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: 2324
  • Accession #: P35916
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Angiogenesis Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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