Human Angiogenesis Array (20 targets) (SARB0019)
- SKU:
- SARB0019
- Product Type:
- Protein Array
- Analytes:
- Angiogenin
- Analytes:
- EGF
- Analytes:
- ENA-78 (CXCL5)
- Analytes:
- bFGF
- Analytes:
- GRO alpha/beta/gamma
- Analytes:
- IFN-gamma
- Analytes:
- IGF-1
- Analytes:
- IL-6
- Analytes:
- IL-8 (CXCL8)
- Analytes:
- Leptin
- Analytes:
- MCP-1 (CCL2)
- Analytes:
- PDGF-BB
- Analytes:
- PLGF
- Analytes:
- RANTES (CCL5)
- Analytes:
- TGF beta 1
- Analytes:
- TIMP-1
- Analytes:
- TIMP-2
- Analytes:
- Thrombopoietin (TPO)
- Analytes:
- VEGF-A
- Analytes:
- VEGF-D
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0019 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | ANG, CCL2, CCL5, CXCL1, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2|FIGF, IFNG, IGF1, IL6, LEP, PDGFB, PGF, TGFB1, THPO, TIMP1, TIMP2, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| EGF
| ENA-78 (CXCL5)
| bFGF
| GRO alpha/beta/gamma
|
IFN-gamma
| IGF-1
| IL-6
| IL-8 (CXCL8)
| Leptin
|
MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
| VEGF-A
| VEGF-D
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
Related Products
Human Angiogenesis Array (23 targets) (SARB0020)
system_update_altDatasheetOverviewProduct Sku:SARB00
Human Angiogenesis Array (43 targets) (SARB0018)
system_update_altDatasheetOverviewProduct Sku:SARB00
Mouse Angiogenesis Array (24 targets) (SARB0072)
system_update_altDatasheetOverviewProduct Sku:SARB00
Human Cancer Discovery Array (20 targets) (SARB0028)
system_update_altDatasheetOverviewProduct Sku:SARB00
Human Periodontal Disease Array (20 targets) (SARB0061)
system_update_altDatasheetOverviewProduct Sku:SARB00