Human Angiogenesis Array (20 targets) (SARB0019)

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SKU:
SARB0019
Product Type:
Protein Array
Analytes:
Angiogenin
Analytes:
EGF
Analytes:
ENA-78 (CXCL5)
Analytes:
bFGF
Analytes:
GRO alpha/beta/gamma
Analytes:
IFN-gamma
Analytes:
IGF-1
Analytes:
IL-6
Analytes:
IL-8 (CXCL8)
Analytes:
Leptin
Analytes:
MCP-1 (CCL2)
Analytes:
PDGF-BB
Analytes:
PLGF
Analytes:
RANTES (CCL5)
Analytes:
TGF beta 1
Analytes:
TIMP-1
Analytes:
TIMP-2
Analytes:
Thrombopoietin (TPO)
Analytes:
VEGF-A
Analytes:
VEGF-D
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0019
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:ANG, CCL2, CCL5, CXCL1, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2|FIGF, IFNG, IGF1, IL6, LEP, PDGFB, PGF, TGFB1, THPO, TIMP1, TIMP2, TPO, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Angiogenin
  • Gene Symbol: ANG
  • Gene ID: 283
  • Accession #: P03950
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
ENA-78 (CXCL5)
  • Gene Symbol: CXCL5
  • Gene ID: 6374
  • Accession #: P42830
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 2247
  • Accession #: P09038
GRO alpha/beta/gamma
  • Gene Symbol: CXCL1/CXCL2/CXCL3
  • Gene ID: 2919/2920/2921
  • Accession #: P09341/P19875/P19876
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-8 (CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
Leptin
  • Gene Symbol: LEP
  • Gene ID: 3952
  • Accession #: P41159
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: 7066
  • Accession #: P40225
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: 2277
  • Accession #: O43915
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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