Enzymes Recombinant Proteins
Human AMD1 Recombinant Protein (RPPB1399)
- SKU:
- RPPB1399
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P17707
- Research Area:
- Enzymes
Description
| Product Name: | Human AMD1 Recombinant Protein |
| Product Code: | RPPB1399 |
| Size: | 5µg |
| Species: | Human |
| Target: | AMD1 |
| Synonyms: | Adenosylmethionine decarboxylase 1, S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, S-adenosylmethionine decarboxylase 1, SAMDC, AMD, EC 4.1.1.50. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The AMD1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GSSHHHHHH SSGLVPRGSH MGSHMSSMFV SKRRFILKTC GTTLLLKALV PLLKLARDYS GFDSIQSFFY SRKNFMKPSH QGYPHRNFQE EIEFLNAIFP NGAAYCMGRM NSDCWYLYTL DFPESRVISQ PDQTLEILMS ELDPAVMDQF YMKDGVTAKD VTRESGIRDL IPGSVIDATM FNPCGYSMNG MKSDGTYWTI HITPEPEFSY VSFETNLSQT SYDDLIRKVV EVFKPGKFVT TLFVNQSSKC RTVLASPQKI EGFKRLDCQS AMFNDYNFVF TSFAKKQQQQ QS |
Adenosylmethionine decarboxylase proenzyme (AMD1) is synthesized originally as an inactive proenzyme. Putrescine stimulates both the proenzyme processing and the catalytic activity. The catalytic activity is inhibited by iodoacetic acid. The active enzyme formation entails a self-maturation process in which the active site pyruvoyl group is produced from an internal serine residue using an autocatalytic post-translational modification.
AMD1 Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (68-334) and having a molecular mass of 33.4 kDa.AMD1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AMD1: an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Two alternatively spliced transcript variants that encode different proteins have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.[provided by RefSeq, Jul 2010] |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; Lyase; Amino Acid Metabolism - cysteine and methionine; EC 4.1.1.50 Chromosomal Location of Human Ortholog: 6q21 Cellular Component: cytosol Molecular Function:adenosylmethionine decarboxylase activity Biological Process: S-adenosylmethioninamine biosynthetic process; spermidine biosynthetic process; spermine biosynthetic process; polyamine metabolic process |
| NCBI Summary: | This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013] |
| UniProt Code: | P17707 |
| NCBI GenInfo Identifier: | 116241324 |
| NCBI Gene ID: | 262 |
| NCBI Accession: | P17707.2 |
| UniProt Secondary Accession: | P17707,Q5VXN4, Q5VXN6, Q9BWK4, E1P5F7, |
| UniProt Related Accession: | P17707 |
| Molecular Weight: | 21,301 Da |
| NCBI Full Name: | S-adenosylmethionine decarboxylase proenzyme |
| NCBI Synonym Full Names: | adenosylmethionine decarboxylase 1 |
| NCBI Official Symbol: | AMD1�� |
| NCBI Official Synonym Symbols: | AMD; SAMDC; ADOMETDC�� |
| NCBI Protein Information: | S-adenosylmethionine decarboxylase proenzyme; S-adenosylmethionine decarboxylase 1 |
| UniProt Protein Name: | S-adenosylmethionine decarboxylase proenzyme |
| Protein Family: | S-adenosylmethionine decarboxylase proenzyme |
| UniProt Gene Name: | AMD1�� |
| UniProt Entry Name: | DCAM_HUMAN |
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