Enzymes Recombinant Proteins

Human ALKBH2 Recombinant Protein (RPPB1397)

(No reviews yet) Write a Review
SKU:
RPPB1397
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q6NS38
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ALKBH2 Recombinant Protein
Product Code:RPPB1397
Size:20µg
Species:Human
Target:ALKBH2
Synonyms:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2, Oxy DC1, ALKBH2, ABH2, FLJ99103, MGC90512.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:ALKBH2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K

ALKBH2 is a member of the ALKB family.ALKBH2 is expressed in the heart, colon, liver, testis, ovary, prostate and the small intestine. ALKBH2 uses iron as a cofactor and acts as a dioxygenase which catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine.

ALKBH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31.4kDa.ALKBH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ALKBH2: Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA repair, damage; EC 1.14.11.33; Oxidoreductase; Hydrolase; Demethylase

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function:ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity

Biological Process: DNA dealkylation; DNA repair

NCBI Summary:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
UniProt Code:Q6NS38
NCBI GenInfo Identifier:74736661
NCBI Gene ID:121642
NCBI Accession:Q6NS38.1
UniProt Related Accession:Q6NS38
Molecular Weight:
NCBI Full Name:DNA oxidative demethylase ALKBH2
NCBI Synonym Full Names:alkB homolog 2, alpha-ketoglutarate dependent dioxygenase
NCBI Official Symbol:ALKBH2��
NCBI Official Synonym Symbols:ABH2��
NCBI Protein Information:DNA oxidative demethylase ALKBH2
UniProt Protein Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
UniProt Synonym Protein Names:Alkylated DNA repair protein alkB homolog 2; DNA oxidative demethylase ALKBH2; Oxy DC1
Protein Family:Alkane 1-monooxygenase
UniProt Gene Name:ALKBH2��
UniProt Entry Name:ALKB2_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products