Enzymes Recombinant Proteins

Human AKR7A3 Recombinant Protein (RPPB5799)

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RPPB5799

Description

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Product Name:Human AKR7A3 Recombinant Protein
Product Code:RPPB5799
Size:10µg
Species:Human
Target:AKR7A3
Synonyms:AFAR2, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2, AFB1-AR 2, AKR7A3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear colorless solution.
Formulation:The AKR7A3 solution contains 20mM Tris-HCl pH-8, 0.1M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR
Biological Activity:Specific activity: approximately < 0.1 units/mg.Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.

AKR7A3, takes part in the detoxification of aldehydes and ketones.AKR7A3 reduces the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. AKR7A3 participates in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

AKR7A3 Human Recombinant fused to a 39 amino acids His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 370 amino acids (1-331 a.a.) and having a molecular mass of 41.6 kDa. The AKR7A3 is fused to a 39 amino acid His tag at n-terminal and purified by proprietary chromatographic techniques.

UniProt Protein Function:AKR7A3: Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytosol

Molecular Function:aldo-keto reductase activity; electron carrier activity; protein binding

Biological Process: aldehyde metabolic process; xenobiotic metabolic process

NCBI Summary:Aldo-keto reductases, such as AKR7A3, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM, Apr 2004]
UniProt Code:O95154
NCBI GenInfo Identifier:13123960
NCBI Gene ID:22977
NCBI Accession:O95154.2
UniProt Secondary Accession:O95154,Q86SR4, Q8IVN6, Q8N5V6, Q8TAX1, Q9NUC3,
UniProt Related Accession:O95154
Molecular Weight:37,206 Da
NCBI Full Name:Aflatoxin B1 aldehyde reductase member 3
NCBI Synonym Full Names:aldo-keto reductase family 7 member A3
NCBI Official Symbol:AKR7A3��
NCBI Official Synonym Symbols:AFAR2��
NCBI Protein Information:aflatoxin B1 aldehyde reductase member 3
UniProt Protein Name:Aflatoxin B1 aldehyde reductase member 3
UniProt Synonym Protein Names:AFB1 aldehyde reductase 2; AFB1-AR 2
Protein Family:Aflatoxin B1 aldehyde reductase
UniProt Gene Name:AKR7A3��
UniProt Entry Name:ARK73_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95154
Research Area:
Enzymes
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