Enzymes Recombinant Proteins
Human AKR1D1 Recombinant Protein (RPPB5797)
- SKU:
- RPPB5797
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P51857
- Research Area:
- Enzymes
Description
| Product Name: | Human AKR1D1 Recombinant Protein |
| Product Code: | RPPB5797 |
| Size: | 20µg |
| Species: | Human |
| Target: | AKR1D1 |
| Synonyms: | 3-oxo-5-beta-steroid 4-dehydrogenase, Aldo-keto reductase family 1 member D1, Delta(4)-3-ketosteroid 5-beta-reductase, Delta(4)-3-oxosteroid 5-beta-reductase, KR1D1, SRD5B1, CBAS2, 3o5bred. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The AKR1D1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY |
Aldo-keto reductase family 1 member D1 (AKR1D1) belongs to the AKR superfamily. The AKR family proteins are soluble NADPH oxidoreductases, which have vital roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is also responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones that carry a delta (4)-3-1 structure. AKR1D1 is highly expressed in the liver, colon and testis. Deficiency of the AKR1D1 enzyme may contribute to hepatic dysfunction.
AKR1D1 Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�346 amino acids (1-326 a.a.) and having a molecular mass of 39.5kDa. The AKR1D1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AKR1D1: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. Defects in AKR1D1 are the cause of congenital bile acid synthesis defect type 2 (CBAS2); also known as cholestasis with delta(4)-3-oxosteroid 5-beta-reductase deficiency. Patients with this liver disease show absence or low levels of chenodeoxycholic acid and cholic acid in plasma and urine. Belongs to the aldo/keto reductase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - primary bile acid biosynthesis; Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.3.1.3; Lipid Metabolism - C21-steroid hormone Chromosomal Location of Human Ortholog: 7q32-q33 Cellular Component: cytosol Molecular Function:aldo-keto reductase activity; steroid binding Biological Process: androgen metabolic process; bile acid biosynthetic process; C21-steroid hormone metabolic process; cholesterol catabolic process; digestion Disease: Bile Acid Synthesis Defect, Congenital, 2 |
| NCBI Summary: | The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P51857 |
| NCBI GenInfo Identifier: | 1703007 |
| NCBI Gene ID: | 6718 |
| NCBI Accession: | P51857.1 |
| UniProt Secondary Accession: | P51857,A1L4P6, A8K060, B4DPN3, B4DPN8, |
| UniProt Related Accession: | P51857 |
| Molecular Weight: | 32,890 Da |
| NCBI Full Name: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| NCBI Synonym Full Names: | aldo-keto reductase family 1, member D1 |
| NCBI Official Symbol: | AKR1D1�� |
| NCBI Official Synonym Symbols: | CBAS2; SRD5B1; 3o5bred�� |
| NCBI Protein Information: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Protein Name: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Synonym Protein Names: | Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase |
| Protein Family: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Gene Name: | AKR1D1�� |
| UniProt Entry Name: | AK1D1_HUMAN |
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