Enzymes Recombinant Proteins

Human AKAP7 Recombinant Protein (RPPB2435)

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RPPB2435

Description

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Product Name:Human AKAP7 Recombinant Protein
Product Code:RPPB2435
Size:10µg
Species:Human
Target:AKAP7
Synonyms:A kinase (PRKA) anchor protein 7, A-kinase anchor protein 18 kDa, A-kinase anchor protein 9, kDa PRKA7 isoforms alpha/beta, PRKA7 isoform gamma, AKAP15, AKAP18.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The AKAP7 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 50mM NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKN KPGEGSSVKT EAADQNGNDN ENNRK

AKAP7 is a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins which bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular sections. AKAP7 is expressed in heart, brain, pancreas, lung and skeletal muscle. AKAP7 binds PKA to the plasma membrane, and allows efficient coupling to the L-type calcium channel.

AKAP7 Human Recombinant produced in E. coli is a single polypeptide chain containing 105 amino acids (1-81) and having a molecular mass of 11.5kDa.AKAP7 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:AKAP7 iso1: Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane- associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6q23.2

Cellular Component: cytosol

Molecular Function:protein binding; protein kinase A binding

Biological Process: protein localization

NCBI Summary:This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Apr 2011]
UniProt Code:O43687
NCBI GenInfo Identifier:25108878
NCBI Gene ID:9465
NCBI Accession:O43687.4
UniProt Secondary Accession:O43687,Q5TBR9, Q5TBS0, Q9HCZ8, Q9P0G4, A8K2K6,
UniProt Related Accession:Q9P0M2,O43687
Molecular Weight:11kDa
NCBI Full Name:A-kinase anchor protein 7 isoforms alpha and beta
NCBI Synonym Full Names:A-kinase anchoring protein 7
NCBI Official Symbol:AKAP7��
NCBI Official Synonym Symbols:AKAP15; AKAP18��
NCBI Protein Information:A-kinase anchor protein 7 isoform gamma; A-kinase anchor protein 7
UniProt Protein Name:A-kinase anchor protein 7 isoforms alpha and beta
UniProt Synonym Protein Names:A-kinase anchor protein 18 kDa; AKAP 18; Protein kinase A-anchoring protein 7 isoforms alpha/beta; PRKA7 isoforms alpha/beta
Protein Family:A-kinase anchor protein
UniProt Gene Name:AKAP7��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O43687
Research Area:
Enzymes
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