Enzymes Recombinant Proteins

Human AK1 Recombinant Protein (RPPB2428)

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SKU:
RPPB2428
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00568
Research Area:
Enzymes

Description

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Product Name:Human AK1 Recombinant Protein
Product Code:RPPB2428
Size:25µg
Species:Human
Target:AK1
Synonyms:Adenylate kinase isoenzyme 1, AK 1, ATP-AMP transphosphorylase 1, Myokinase, AK1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The AK1 protein solution (1mg/ml) contains 20mM Tris-HCl pH-7.5 and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK
Biological Activity:Specific activity: > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25�C.

AK1 is a small ubiquitous enzyme which is essential for maintenance and cell growth. It is involved in the regulation of adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. The AK1 protein is found in the cytosol of skeletal muscle, brain and erythrocytes. Defects in the AK1 gene are the cause of a form of hemolytic anemia.

AK1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 214 amino acids (1-194 a.a.) and having a molecular mass of 23.7kDa. The AK1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.
UniProt Protein Details:

Protein type:EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.3

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; cytosol

Molecular Function:adenylate kinase activity; nucleoside diphosphate kinase activity

Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process

Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To

NCBI Summary:This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:P00568
NCBI GenInfo Identifier:20178288
NCBI Gene ID:203
NCBI Accession:P00568.3
UniProt Secondary Accession:P00568,Q9BVK9, Q9UQC7,
UniProt Related Accession:P00568
Molecular Weight:21,635 Da
NCBI Full Name:Adenylate kinase isoenzyme 1
NCBI Synonym Full Names:adenylate kinase 1
NCBI Official Symbol:AK1��
NCBI Official Synonym Symbols:HTL-S-58j��
NCBI Protein Information:adenylate kinase isoenzyme 1
UniProt Protein Name:Adenylate kinase isoenzyme 1
UniProt Synonym Protein Names:ATP-AMP transphosphorylase 1
Protein Family:Adenylate kinase
UniProt Gene Name:AK1��
UniProt Entry Name:KAD1_HUMAN
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