Human AFAP1 Recombinant Protein (RPPB2708)

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RPPB2708

Description

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Product Name:Human AFAP1 Recombinant Protein
Product Code:RPPB2708
Size:10µg
Species:Human
Target:AFAP1
Synonyms:Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP, Actin Filament Associated Protein 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The AFAP1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGCSGPVDSE CPPPPSSPVH KAELEKKLSS ERPSSDGEGV VENGITTCNG KEQVKRKKSS KSEAKGTVSK VTGKKITKII SLGKKKPSTD EQTSSAEEDV PTCGYLNVLS NSRWRERWCR VKDNKLIFHK DRTDLKTHIV SIPLRGCEVI PGLDCKHPLT FRLLRNGQEV AVLEASSSED MGRWIGILLA ETGSSTDPEA LHYDYIDVEM SASVIQTAKQ TFCFMNRRVI SANPYLGGTS NGYAHPSGTA LHYDDVPCIN GSLRGKKPPV ASNGVTGKGK TLSSQPKKAD PAAVVKRTGS NAAQYKYGKN RVEADAKRLQ TKEEELLKRK EALRNRLAQL

Actin Filament Associated Protein 1 (AFAP1) is a Src binding partner. AFAP1 is a possible modulator of actin filament integrity in response to cellular signals, and is also playing a role as an adaptor protein by connecting Src family members to actin filaments. AFAP1 takes partin the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

AFAP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 360 amino acids (250-588) and having a molecular mass of 39.2 kDa.AFAP1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:AFAP: Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function:actin binding

Biological Process: regulation of signal transduction; regulation of cytoskeleton organization and biogenesis

NCBI Summary:The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:Q8N556
NCBI GenInfo Identifier:166919564
NCBI Gene ID:60312
NCBI Accession:Q8N556.2
UniProt Secondary Accession:Q8N556,Q59EY5, Q9HBY1, A8K442, B4DMU2, E9PDT7,
UniProt Related Accession:Q8N556
Molecular Weight:730
NCBI Full Name:Actin filament-associated protein 1
NCBI Synonym Full Names:actin filament associated protein 1
NCBI Official Symbol:AFAP1��
NCBI Official Synonym Symbols:AFAP; AFAP110; AFAP-110��
NCBI Protein Information:actin filament-associated protein 1; 110 kDa actin filament-associated protein; actin filament-associated protein, 110 kDa
UniProt Protein Name:Actin filament-associated protein 1
UniProt Synonym Protein Names:110 kDa actin filament-associated protein; AFAP-110
Protein Family:Actin filament-associated protein
UniProt Gene Name:AFAP1��
UniProt Entry Name:AFAP1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8N556
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