Enzymes Recombinant Proteins

Human ADK Recombinant Protein (RPPB2426)

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RPPB2426

Description

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Product Name:Human ADK Recombinant Protein
Product Code:RPPB2426
Size:10µg
Species:Human
Target:ADK
Synonyms:Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase, Testicular Tissue Protein Li 14, EC 2.7.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The ADK protein solution (0.5mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH8.0), 1mM DTT, 1mM EDTA�& 50mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKARVCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPDFH
Biological Activity:Specific activity is > 30 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Adenosine Kinase is an abundant enzyme in mammalian tissues which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thus is as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has extensive effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme take a crucial pharmacological part in growing intravascular adenosine concentrations and acting as anti-inflammatory agents.

ADK produced in E.Coli is a single, non-glycosylated polypeptide chain containing 362 amino acids (22-362a.a.) and having a molecular mass of 40.5kDa.ADK is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.20

Chromosomal Location of Human Ortholog: 10q22|10q11-q24

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function:adenosine kinase activity

Biological Process: purine salvage; ribonucleoside monophosphate biosynthetic process

Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency

NCBI Summary:This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:P55263
NCBI GenInfo Identifier:6840802
NCBI Gene ID:132
NCBI Accession:P55263.2
UniProt Secondary Accession:P55263,O00741, O00742, Q16710, Q5JQ10, Q5JQ11, Q9BTN2 B7Z783, B7Z800,
UniProt Related Accession:P55263
Molecular Weight:36,580 Da
NCBI Full Name:Adenosine kinase
NCBI Synonym Full Names:adenosine kinase
NCBI Official Symbol:ADK��
NCBI Official Synonym Symbols:AK��
NCBI Protein Information:adenosine kinase
UniProt Protein Name:Adenosine kinase
UniProt Synonym Protein Names:Adenosine 5'-phosphotransferase
UniProt Gene Name:ADK��
UniProt Entry Name:ADK_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P55263
Research Area:
Enzymes
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