Enzymes Recombinant Proteins

Human ADH1C Recombinant Protein (RPPB1354)

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SKU:
RPPB1354
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00326
Research Area:
Enzymes

Description

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Product Name:Human ADH1C Recombinant Protein
Product Code:RPPB1354
Size:20µg
Species:Human
Target:ADH1C
Synonyms:Alcohol dehydrogenase 1C (class I) gamma polypeptide, Alcohol dehydrogenase subunit gamma, alcohol dehydrogenase 3 (class I) gamma polypeptide, ADH gamma subunit, aldehyde reductase, ADH3, EC 1.1.1.1, EC 1.1.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ADH1C solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSTAGK VIKCKAAVLW ELKKPFSIEE VEVAPPKAHE VRIKMVAAGI CRSDEHVVSG NLVTPLPVIL GHEAAGIVES VGEGVTTVKP GDKVIPLFTP QCGKCRICKN PESNYCLKND LGNPRGTLQD GTRRFTCSGK PIHHFVGVST FSQYTVVDEN AVAKIDAASP LEKVCLIGCG FSTGYGSAVK VAKVTPGSTC AVFGLGGVGL SVVMGCKAAG AARIIAVDIN KDKFAKAKEL GATECINPQD YKKPIQEVLK EMTDGGVDFS FEVIGRLDTM MASLLCCHEA CGTSVIVGVP PDSQNLSINP MLLLTGRTWK GAIFGGFKSK ESVPKLVADF MAKKFSLDAL ITNILPFEKI NEGFDLLRSG KSIRTVLTF

ADH1C is a member of the zinc-containing alcohol dehydrogenase family which metabolizes a large assortment of substrates, such as ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. ADH1 is a monomorphic and a key factor in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood.

ADH1C Human Recombinant produced in E. coli is a single polypeptide chain containing 399 amino acids (1-375) and having a molecular mass of 42.4 kDa.ADH1C is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ADH1C: class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; Lipid Metabolism - fatty acid; Mitochondrial; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol; nucleoplasm; plasma membrane

Molecular Function:alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; retinol dehydrogenase activity

Biological Process: ethanol oxidation

Disease: Alcohol Dependence; Parkinson Disease, Late-onset

NCBI Summary:This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]
UniProt Code:P00326
NCBI GenInfo Identifier:113398
NCBI Gene ID:126
NCBI Accession:P00326.2
UniProt Secondary Accession:P00326,Q4PJ18, Q5WRV0, Q6LBW4, Q6NWV0, Q6NZA7,
UniProt Related Accession:P00326
Molecular Weight:40kDa
NCBI Full Name:Alcohol dehydrogenase 1C
NCBI Synonym Full Names:alcohol dehydrogenase 1C (class I), gamma polypeptide
NCBI Official Symbol:ADH1C��
NCBI Official Synonym Symbols:ADH3��
NCBI Protein Information:alcohol dehydrogenase 1C
UniProt Protein Name:Alcohol dehydrogenase 1C
UniProt Synonym Protein Names:Alcohol dehydrogenase subunit gamma
Protein Family:Alcohol dehydrogenase
UniProt Gene Name:ADH1C��
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