ADAMTS4 (ADAM Metallopeptidase With Thrombospondin Type 1 Motif 4) is a Protein Coding gene. ADAMTS4 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. This protein functions as a metalloproteinase, degrading extracellular matrix proteins. The encoded protein is secreted and may play a role in tissue remodeling. ADAMTS4 is located on chromosome 6p21.3. The encoded protein is secreted and may play a role in tissue remodeling. ADAMTS4 has been shown to play a role in diseases such as atherosclerosis, cancer, and arthritis.
Product Name:
Human ADAMTS4 ELISA Kit
Product Code:
HUFI01626
Size:
96 Assays
Alias:
ADAMTS4, ADAMTS2, ADMP-1, ADAM-TS 4, ADAMTS-2, ADAM-TS4, ADAMTS-4, Aggrecanase 1, a disintegrin-like and metalloprotease, reprolysin type with thrombospondintype 1 motif, 4, ADAM metallopeptidase with thrombospondin type 1 motif, 4, A disintegrin and metalloproteinase with thrombospondin motifs 4
Detection method:
Sandwich ELISA, Double Antibody
Application:
This immunoassay kit allows for the in vitro quantitative determination of Human ADAMTS4 concentrations in serum plasma and other biological fluids.
Sensitivity:
37.5pg/ml
Range:
62.5-4000pg/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human ADAMTS4 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ADAMTS4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
96
EDTA plasma(n=5)
92-99
95
UFH plasma(n=5)
90-104
98
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ADAMTS4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-104%
89-104%
86-97%
EDTA plasma(n=5)
84-100%
84-101%
85-100%
UFH plasma(n=5)
80-98%
83-99%
86-98%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu--Ala-393' site.
UniProt Protein Details:
Protein type:Motility/polarity/chemotaxis; Secreted, signal peptide; Protease; Secreted; EC 3.4.24.82
Chromosomal Location of Human Ortholog:
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ADAMTS4, ADAMTS2, ADMP-1, ADAM-TS 4, ADAMTS-2, ADAM-TS4, ADAMTS-4, Aggrecanase 1, a disintegrin-like and metalloprotease, reprolysin type with thrombospondintype 1 motif, 4, ADAM metallopeptidase with thrombospondin type 1 motif, 4, A disintegrin and
