Enzymes Recombinant Proteins
Human ADA Recombinant Protein (RPPB5787)
- SKU:
- RPPB5787
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00813
- Research Area:
- Enzymes
Description
| Product Name: | Human ADA Recombinant Protein |
| Product Code: | RPPB5787 |
| Size: | 10µg |
| Species: | Human |
| Target: | ADA |
| Synonyms: | Adenosine deaminase, Adenosine aminohydrolase, ADA1, EC 3.5.4.4. |
| Source: | Escherichia Coli |
| Physical Appearance: | ADA is supplied as a sterile filtered clear solution. |
| Formulation: | ADA protein 0.5mg/ml is supplied in 20mM Tris-HCL, pH-8, 1mM DTT and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL |
| Biological Activity: | Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C. |
ADA catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. ADA has a vital part in purine metabolism and in adenosine homeostasis. ADA performs as a positive regulator of T-cell coactivation, by binding DPP4 which regulates lymphocyte-epithelial cell adhesion.
ADA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 383 amino acids (1-363) and having a molecular mass of 42.9 kDa.The ADA is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm. |
| UniProt Protein Details: | Protein type:Hydrolase; Nucleotide Metabolism - purine; EC 3.5.4.4 Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell surface; cytoplasm; cytosol; external side of plasma membrane; lysosome; membrane; plasma membrane Molecular Function:adenosine deaminase activity; protein binding; zinc ion binding Biological Process: adenosine catabolic process; hypoxanthine salvage; inosine biosynthetic process; negative regulation of adenosine receptor signaling pathway; purine nucleotide salvage; purine salvage; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00813 |
| NCBI GenInfo Identifier: | 113339 |
| NCBI Gene ID: | 100 |
| NCBI Accession: | P00813.3 |
| UniProt Secondary Accession: | P00813,Q53F92, Q6LA59, |
| UniProt Related Accession: | P00813 |
| Molecular Weight: | 40,764 Da |
| NCBI Full Name: | Adenosine deaminase |
| NCBI Synonym Full Names: | adenosine deaminase |
| NCBI Official Symbol: | ADA�� |
| NCBI Protein Information: | adenosine deaminase |
| UniProt Protein Name: | Adenosine deaminase |
| UniProt Synonym Protein Names: | Adenosine aminohydrolase |
| Protein Family: | ADAM |
| UniProt Gene Name: | ADA�� |
| UniProt Entry Name: | ADA_HUMAN |
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