Growth Factors & Cytokines Recombinant Proteins

Human Activin B Recombinant Protein (RPPB0007)

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SKU:
RPPB0007
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P09529
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human Activin B Recombinant Protein
Product Code:RPPB0007
Size:10µg
Species:Human
Target:Activin B
Synonyms:Inhibin beta B (activin AB beta polypeptide), Inhibin, beta-2, Activin beta-B chain, MGC157939.
Source:Nicotiana benthamiana plant
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from 1mg/ml solution in 0.05M Tris-HCl buffer pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by Analysis by SDS-PAGE.
Amino Acid Sequence:HHHHHHHHHH GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG
Biological Activity:The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 <5ng/ml is required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Inhibins are dimeric peptide hormones produced by female ovarian granulose cells and male Sertoli cells as well as a variety of other tissues. Inhibins have two isoforms, A and B, with the same alpha subunit but different beta subunits. Inhibin A is a dimer of alpha and beta A subunits, inhibin B is a dimer of alpha and beta B subunits.Inhibins are thought to inhibit the production of follicle-stimulating hormone (FSH) by the pituitary gland. In addition, Inhibins are also thought to play a role in the control of gametogenesis, and embryonic and fetal development.

Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

UniProt Protein Function:INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2cen-q13

Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function:cytokine activity; growth factor activity; hormone activity; host cell surface receptor binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; cell development; cell differentiation; cellular response to insulin stimulus; cellular response to starvation; defense response; fat cell differentiation; growth; negative regulation of follicle-stimulating hormone secretion; negative regulation of hepatocyte growth factor biosynthetic process; negative regulation of insulin secretion; oocyte development; ovarian follicle development; positive regulation of follicle-stimulating hormone secretion; regulation of apoptosis; regulation of MAPKKK cascade; response to mechanical stimulus

NCBI Summary:The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]
UniProt Code:P09529
NCBI GenInfo Identifier:1708437
NCBI Gene ID:3625
NCBI Accession:P09529.2
UniProt Secondary Accession:P09529,Q53T31, Q8N1D3,
UniProt Related Accession:P09529
Molecular Weight:45,122 Da
NCBI Full Name:Inhibin beta B chain
NCBI Synonym Full Names:inhibin beta B
NCBI Official Symbol:INHBB��
NCBI Protein Information:inhibin beta B chain
UniProt Protein Name:Inhibin beta B chain
UniProt Synonym Protein Names:Activin beta-B chain
Protein Family:Inhibin
UniProt Gene Name:INHBB��
UniProt Entry Name:INHBB_HUMAN
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