Growth Factors & Cytokines Recombinant Proteins

Human Activin-A Recombinant Protein (RPPB0002)

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SKU:
RPPB0002
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P08476
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human Activin-A Recombinant Protein
Product Code:RPPB0002
Size:5µg
Species:Human
Target:Activin-A
Synonyms:Inhba, Inhibin beta A, FSH releasing protein.
Source:Nicotiana benthamiana
Physical Appearance:Lyophilized freeze dried powder.
Formulation:Active form Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 50mM Tris-HCl pH-7.4
Solubility:INHBA protein should be reconstituted in distilled water to a concentration of 50 ug /ml. Due to the protein nature, dimmers and multimers may be observed.
Stability:For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Purity:Greater than 98% as obsereved by SDS-PAGE.
Amino Acid Sequence:HHHHHHGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
Biological Activity:The biological activity of INHBA is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation). ED50<5ng/ml.

Activins are homodimers or heterodimers of the different Beta subunit isoforms, part of the TGFBeta family. Mature Activin A has two 116 amino acids residues BetaA subunits (BetaA-BetaA). Activin displays an extensive variety of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins takes part in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells that are identified to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.

Active form Activin-A Human Recombinant produced in Plant is a homodimeric, glycosylated, polypeptide chain containing 2 x 116 amino acids and having a molecular weight of 27.4kDa.The Active form Activin-A is fused to a 6-His tag at N-terminus and purified by standard chromatographic techniques.

UniProt Protein Function:INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: extracellular region

Molecular Function:cytokine activity; growth factor activity; hormone activity; identical protein binding; peptide hormone binding; protein binding; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; cell cycle arrest; cell development; cell differentiation; cell surface receptor linked signal transduction; cell-cell signaling; defense response; G1/S transition of mitotic cell cycle; hair follicle development; hemoglobin biosynthetic process; hemopoietic progenitor cell differentiation; male gonad development; negative regulation of B cell differentiation; negative regulation of cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of interferon-gamma biosynthetic process; negative regulation of macrophage differentiation; negative regulation of phosphorylation; odontogenesis; ovarian follicle development; palate development; positive regulation of cellular protein metabolic process; positive regulation of erythrocyte differentiation; positive regulation of follicle-stimulating hormone secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; progesterone secretion; regulation of follicle-stimulating hormone secretion; regulation of MAPKKK cascade; regulation of transcription from RNA polymerase II promoter; response to drug; striatal medium spiny neuron differentiation

NCBI Summary:This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of the dimeric activin and inhibin protein complexes. These complexes activate and inhibit, respectively, follicle stimulating hormone secretion from the pituitary gland. The encoded protein also plays a role in eye, tooth and testis development. Elevated expression of this gene may be associated with cancer cachexia in human patients. [provided by RefSeq, Aug 2016]
UniProt Code:P08476
NCBI GenInfo Identifier:124279
NCBI Gene ID:3624
NCBI Accession:P08476.2
UniProt Secondary Accession:P08476,Q14599,
UniProt Related Accession:P08476
Molecular Weight:47,442 Da
NCBI Full Name:Inhibin beta A chain
NCBI Synonym Full Names:inhibin beta A subunit
NCBI Official Symbol:INHBA��
NCBI Official Synonym Symbols:EDF; FRP��
NCBI Protein Information:inhibin beta A chain
UniProt Protein Name:Inhibin beta A chain
UniProt Synonym Protein Names:Activin beta-A chain; Erythroid differentiation protein; EDF
Protein Family:Inhibin
UniProt Gene Name:INHBA��
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