Enzymes Recombinant Proteins

Human ACOT8 Recombinant Protein (RPPB5775)

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RPPB5775

Description

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Product Name:Human ACOT8 Recombinant Protein
Product Code:RPPB5775
Size:20µg
Species:Human
Target:ACOT8
Synonyms:Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2, Acyl-CoA thioesterase 8, Choloyl-coenzyme A thioesterase, HIV-Nef-associated acyl-CoA thioesterase, PTE-2, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1 Thioesterase II, ACTEIII, hACTEIII, the, ACOT8.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ACOT8 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL

Acyl-CoA Thioesterase 8 (ACOT8) is a group of enzymes which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), granting the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT8 mediate Nef-induced down-regulation of CD4. ACOT8 contends with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). ACOT8 prefers medium-length fatty acyl-CoAs.

ACOT8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 342 amino acids (1-319) and having a molecular mass of 38.3kDa. ACOT8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ACOT8: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs. May be involved in the metabolic regulation of peroxisome proliferation. Belongs to the C/M/P thioester hydrolase family.
UniProt Protein Details:

Protein type:EC 3.1.2.27; Hydrolase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: peroxisomal matrix

Molecular Function:acetyl-CoA hydrolase activity; acyl-CoA hydrolase activity; CoA hydrolase activity; palmitoyl-CoA hydrolase activity; protein binding; receptor binding

Biological Process: acyl-CoA metabolic process; bile acid biosynthetic process; dicarboxylic acid catabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; negative regulation of CD4 biosynthetic process; peroxisome fission

NCBI Summary:The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
UniProt Code:O14734
NCBI GenInfo Identifier:14285685
NCBI Gene ID:10005
NCBI Accession:O14734.1
UniProt Secondary Accession:O14734,O15261, Q17RX4,
UniProt Related Accession:O14734
Molecular Weight:35,914 Da
NCBI Full Name:Acyl-coenzyme A thioesterase 8
NCBI Synonym Full Names:acyl-CoA thioesterase 8
NCBI Official Symbol:ACOT8��
NCBI Official Synonym Symbols:hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III��
NCBI Protein Information:acyl-coenzyme A thioesterase 8
UniProt Protein Name:Acyl-coenzyme A thioesterase 8
UniProt Synonym Protein Names:Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE
Protein Family:Acyl-coenzyme A thioesterase
UniProt Gene Name:ACOT8��
UniProt Entry Name:ACOT8_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O14734
Research Area:
Enzymes
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