Description
| Product Name: | Human ACOT13 Recombinant Protein |
| Product Code: | RPPB1334 |
| Size: | 10µg |
| Species: | Human |
| Target: | ACOT13 |
| Synonyms: | Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, Thioesterase superfamily member 2, ACOT13, THEM2, HT012, MGC4961, PNAS-27, ACOT13. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ACOT13 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN |
Acyl-coenzyme A thioesterase 13 (ACOT13) belongs to the thioesterase subfamily of esterase family. ACOT13 is highly expressed in the kidney with moderate expression in the brain, liver and intestines. ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly having a role in cellular proliferation events. Deletion of a segment of the q arm of chromosome 6 is linked to early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus.
ACOT13 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 160 amino acids (1-140 a.a.) and having a molecular mass of 17.1kDa. The ACOT13 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family. |
| UniProt Protein Details: | Protein type:EC 3.1.2.- Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:acyl-CoA hydrolase activity Biological Process: protein homotetramerization |
| NCBI Summary: | This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009] |
| UniProt Code: | Q9NPJ3 |
| NCBI GenInfo Identifier: | 23396822 |
| NCBI Gene ID: | 55856 |
| NCBI Accession: | Q9NPJ3.1 |
| UniProt Secondary Accession: | Q9NPJ3,O95549, F5H2L4, |
| UniProt Related Accession: | Q9NPJ3 |
| Molecular Weight: | 12,366 Da |
| NCBI Full Name: | Acyl-coenzyme A thioesterase 13 |
| NCBI Synonym Full Names: | acyl-CoA thioesterase 13 |
| NCBI Official Symbol: | ACOT13�� |
| NCBI Official Synonym Symbols: | HT012; THEM2; PNAS-27�� |
| NCBI Protein Information: | acyl-coenzyme A thioesterase 13 |
| UniProt Protein Name: | Acyl-coenzyme A thioesterase 13 |
| UniProt Synonym Protein Names: | Thioesterase superfamily member 2 |
| UniProt Gene Name: | ACOT13�� |
