Natural Proteins Recombinant Proteins

Human A2M Recombinant Protein (RPPB2814)

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SKU:
RPPB2814
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01023
Research Area:
Natural Proteins

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human A2M Recombinant Protein
Product Code:RPPB2814
Size:1mg
Species:Human
Target:A2M
Synonyms:Alpha-2-macroglobulin, Alpha-2-M, A2M, CPAMD5, FWP007, S863-7, alpha 2M, DKFZp779B086.
Source:Human Plasma
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a concentrated solution containing 5mM potassium phosphate buffer, pH 6.5 and 1:1 ratio (w/w) of Glycine.
Solubility:It is recommended to reconstitute the lyophilized A2M in sterile 18M?-cm H2O.
Stability:Lyophilized A2M protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization A2M can be stored at 4°C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0%.

Alpha-2 Macroglobulin is a serine proteases inhibitor, which inhibits coagulation by inactivating thrombin and Kallikrein, it inhibits fibrinolysis by inactivating plasmin and involved in transport. Alpha-2-Macroglobulin is a large plasma protein, which is produced by the liver, it�s composed of 4 identical subunits bound together by -S-S- bonds. A2M is able to inactivate many kinds of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). A2M has a 35 amino acid "bait" region in its structure. Proteinases bind and cleave the �bait� region become bound to A2M. Macrophage receptors recognize the proteinase-A2M complex and clear it from the system. A2M binds to and removes MMP-2 and MMP-9 (active forms of the gelatinase) from the circulation using scavenger receptors on the phagocytes. The levels of Alpha-2-macroglobulin are increased in nephrotic syndrome which is a condition where the kidneys start to leak out some of the smaller blood proteins. Due to its large size, A2-macroglobulin is retained in the bloodstream. Increased production of all proteins causes A2-macroglobulin concentration to increase. Chronic renal failure might lead to amyloid by alpha-2-macroglobulin. A2M is raised in cirrhosis, pregnancy and diabetes.

Human Alpha-2 Macroglobulin is a tetrameric glycoprotein, produced in Human plasma and having a molecular mass of 725 kDa.

UniProt Protein Function:Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
UniProt Protein Details:

Subunit structure: Homotetramer; disulfide-linked. Ref.8 Ref.10

Subcellular location: Secreted Ref.8.

Tissue specificity: Secreted in plasma. Ref.8

Developmental stage: Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Sequence caution: The sequence AAT02228.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAD92851.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

NCBI Summary:Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq]
UniProt Code:P01023
NCBI GenInfo Identifier:112911
NCBI Gene ID:2
NCBI Accession:P01023.1
UniProt Secondary Accession:P01023,Q13677, Q59F47, Q5QTS0, Q68DN2, Q6PIY3, Q6PN97
UniProt Related Accession:P01023,Q9BQ22
Molecular Weight:163,291 Da
NCBI Full Name:Alpha-2-macroglobulin
NCBI Synonym Full Names:alpha-2-macroglobulin
NCBI Official Symbol:A2M��
NCBI Official Synonym Symbols:A2MD; CPAMD5; FWP007; S863-7; DKFZp779B086��
NCBI Protein Information:alpha-2-macroglobulin; alpha-2-M; OTTHUMP00000196829; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Protein Name:Alpha-2-macroglobulin
UniProt Synonym Protein Names:C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Protein Family:Alpha-2-macroglobulin
UniProt Gene Name:A2M��
UniProt Entry Name:A2MG_HUMAN
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