HRP Detection Reagent (Fluorometric) (BA0173)

HRP Detection Reagent:

Assay Genie's fluorometric HRP detection reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red TM) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent product (Ex/Em = 530/590nm) when reacted with H₂O₂ by a peroxidase. The fluorescent product can also be visualized under a light source (530 ± 20 nm).

Applications:

Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red TM) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/590nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm).

HRP Detection Reagent - Key Features

• For both absorbance or fluorescence detection.
• Highly sensitive.
• Convenient and fast.

HRP Detection Reagent Information:

*Ampliflu Red is a trademark of Sigma-Aldrich