Enzymes Recombinant Proteins

HPSE WB Recombinant Protein (RPPB1820)

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RPPB1820

Description

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Product Name:HPSE WB Recombinant Protein
Product Code:RPPB1820
Size:200ng
Target:HPSE WB
Formulation:Concentration: 1?g /mlContent: 100 ngBuffer: LDS-PAGE buffer[140 mM Tris buffer pH 8.5, 10% Glycerol, 2% LDS, 0.015% EDTA, 1.88% (v/v) of 1% Serva Blue G250 and 0.625% (v/v) of 1% Phenol red].

Heparanase is an endo Beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).

Recombinant Heparanase protein HPA1 is produced in CHO cells.The protein is purified by several orthogonal chromatography steps.

UniProt Protein Function:HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.
UniProt Protein Details:

Protein type:EC 3.2.1.166; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; lysosomal membrane; lysosome; extracellular region; nucleus; lipid raft

Molecular Function:protein dimerization activity; syndecan binding; protein binding; beta-glucuronidase activity; heparanase activity

Biological Process: positive regulation of hair follicle development; proteoglycan metabolic process; positive regulation of protein kinase B signaling cascade; glycosaminoglycan catabolic process; heparan sulfate proteoglycan catabolic process; positive regulation of blood coagulation; glycosaminoglycan metabolic process; cell-matrix adhesion; positive regulation of osteoblast proliferation; carbohydrate metabolic process; pathogenesis; regulation of hair follicle development

NCBI Summary:Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q9Y251
NCBI GenInfo Identifier:296434532
NCBI Gene ID:10855
NCBI Accession:Q9Y251.2
UniProt Secondary Accession:Q9Y251,Q53GE5, Q9UL39, A9JIG7, C7F7I3, C7F7I4, E9PCA9 E9PGR1,
UniProt Related Accession:Q9Y251
Molecular Weight:42,791 Da
NCBI Full Name:Heparanase
NCBI Synonym Full Names:heparanase
NCBI Official Symbol:HPSE��
NCBI Official Synonym Symbols:HPA; HPA1; HPR1; HSE1; HPSE1��
NCBI Protein Information:heparanase; endo-glucoronidase; heparanase-1
UniProt Protein Name:Heparanase
UniProt Synonym Protein Names:Endo-glucoronidase; Heparanase-1; Hpa1
Protein Family:HpaII very short patch repair endonuclease
UniProt Gene Name:HPSE��
UniProt Entry Name:HPSE_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Uniprot:
Q9Y251
Research Area:
Enzymes
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