Name: HOXA4 Antibody (PACO56836)
SKU: PACO56836
Availability: OutOfStock

Antibody Name:	HOXA4 Antibody (PACO56836)
Antibody SKU:	PACO56836
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Homeobox protein Hox-A4 protein (23-173AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: HOXA4 antibody at 2.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa.

IHC image of PACO56836 diluted at 1:800 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Synonyms:	Homeobox protein Hox-A4 (Homeobox protein Hox-1.4) (Homeobox protein Hox-1D), HOXA4, HOX1D

UniProt Protein Function:	HOXA4: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'. Belongs to the Antp homeobox family. Deformed subfamily.Protein type: DNA-binding; Transcription factorChromosomal Location of Human Ortholog: 7p15.2Cellular Component: nucleusMolecular Function: sequence-specific DNA binding; transcription factor activityBiological Process: anterior/posterior pattern formation; anatomical structure morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; embryonic skeletal morphogenesis
UniProt Protein Details:
NCBI Summary:	In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q00056
NCBI GenInfo Identifier:	116242514
NCBI Gene ID:	3201
NCBI Accession:	Q00056.3
UniProt Secondary Accession:	Q00056,O43366, A4D180
UniProt Related Accession:	Q00056
Molecular Weight:	34,499 Da
NCBI Full Name:	Homeobox protein Hox-A4
NCBI Synonym Full Names:	homeobox A4
NCBI Official Symbol:	HOXA4
NCBI Official Synonym Symbols:	HOX1; HOX1D
NCBI Protein Information:	homeobox protein Hox-A4; homeo box A4; Dfd-like protein; Hox-1.4-like protein; homeobox protein Hox-1D; homeobox protein Hox-1.4
UniProt Protein Name:	Homeobox protein Hox-A4
UniProt Synonym Protein Names:	Homeobox protein Hox-1.4; Homeobox protein Hox-1D
Protein Family:	Homeobox protein
UniProt Gene Name:	HOXA4
UniProt Entry Name:	HXA4_HUMAN