HMGB4 Antibody (PACO14516)
- SKU:
- PACO14516
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | HMGB4 Antibody (PACO14516) |
| Antibody SKU: | PACO14516 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:1000-1:2000, WB:1:200-1:1000 |
| Species Reactivity: | Human |
| Immunogen: | Fusion protein of human HMGB4 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 231 cells, human fetal liver tissue, Primary antibody: PACO14516(HMGB4 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes. |
| Background: | High mobility group protein B4 is a transcription factor that in humans is encoded by the HMGB4 gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus. |
| Synonyms: | high mobility group box 4 |
| UniProt Protein Function: | HMGB4: Belongs to the HMGB family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1p35.1 Cellular Component: chromosome; nucleus Molecular Function:DNA binding; chromatin binding Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent |
| UniProt Code: | Q8WW32 |
| NCBI GenInfo Identifier: | 387912835 |
| NCBI Gene ID: | 127540 |
| NCBI Accession: | Q8WW32.2 |
| UniProt Secondary Accession: | Q8WW32,Q0QWA4, B2R4X7, |
| UniProt Related Accession: | Q8WW32 |
| Molecular Weight: | 186 |
| NCBI Full Name: | High mobility group protein B4 |
| NCBI Synonym Full Names: | high mobility group box 4 |
| NCBI Official Symbol: | HMGB4 |
| NCBI Official Synonym Symbols: | dJ1007G16.5 |
| NCBI Protein Information: | high mobility group protein B4; HMG2 like |
| UniProt Protein Name: | High mobility group protein B4 |
| Protein Family: | High mobility group protein |
| UniProt Gene Name: | HMGB4 |
| UniProt Entry Name: | HMGB4_HUMAN |
| Antibodies | ELISA Kits |
| HMGB4 Antibody (PACO09746) | Human HMGB4 / High mobility group protein B4 ELISA Kit |
| Human High mobility group protein B4 (HMGB4) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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