Antibody Name:	Hmgb1 Antibody (PACO64271)
Antibody SKU:	PACO64271
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	WB:1:1000-1:5000
Species Reactivity:	Rat
Immunogen:	Peptide sequence from Rat Hmgb1 protein (2-18AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Antigen Affinity purified & Affinity purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: Rat Brain tissue. All lanes: Hmgb1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 kDa. Observed band size: 30 kDa.

Background:

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acid,. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acid, implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid, (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins.

Synonyms:

High mobility group protein B1 (Amphoterin) (Heparin-binding protein p30) (High mobility group protein 1) (HMG-1), Hmgb1, Hmg-1 Hmg1

UniProt Protein Function:	Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide (PubMed:11154118). Bound to RAGE mediates signaling for neuronal outgrowth (PubMed:1885601, PubMed:2461949, PubMed:7592757, PubMed:12183440). May play a role in accumulation of expanded polyglutamine (polyQ) proteins.
NCBI Summary:	heparin binding protein that facilitates neurite outgrowth [RGD, Feb 2006]
UniProt Code:	P63159
NCBI GenInfo Identifier:	6981026
NCBI Gene ID:	25459
NCBI Accession:	NP_037095.1
UniProt Secondary Accession:	P63159,P07155, P27109, P27428, Q548R9,
UniProt Related Accession:	P63159
Molecular Weight:	24,894 Da
NCBI Full Name:	high mobility group protein B1
NCBI Synonym Full Names:	high mobility group box 1
NCBI Official Symbol:	Hmgb1
NCBI Official Synonym Symbols:	Hmg1; Ac2-008
NCBI Protein Information:	high mobility group protein B1
UniProt Protein Name:	High mobility group protein B1
UniProt Synonym Protein Names:	Amphoterin; Heparin-binding protein p30; High mobility group protein 1; HMG-1
Protein Family:	High mobility group protein
UniProt Gene Name:	Hmgb1