HLA-C Antibody (PACO54462)
- SKU:
- PACO54462
Description
| Antibody Name: | HLA-C Antibody (PACO54462) |
| Antibody SKU: | PACO54462 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human HLA class I histocompatibility antigen, Cw-3 α chain protein (200-306AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO54462 at dilution of 1:100. |
 | Immunofluorescence staining of HepG2 cells with PACO54462 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Involved in the presentation of foreign antigens to the immune system. |
| Synonyms: | HLA class I histocompatibility antigen, Cw-3 α chain (MHC class I antigen Cw*3), HLA-C, HLAC |
| UniProt Protein Function: | Involved in the presentation of foreign antigens to the immune system. |
| UniProt Protein Details: | |
| NCBI Summary: | HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04222 |
| NCBI GenInfo Identifier: | 34223716 |
| NCBI Gene ID: | 3107 |
| NCBI Accession: | P04222.2 |
| UniProt Secondary Accession: | P04222,O62883, O78060, O78068, O78069, P30503, Q29743 Q29992, Q29993, Q9GIK8 |
| UniProt Related Accession: | P04222, P10321, P30499, P30501, P30504, P30505, P30508, P30510, Q07000 |
| Molecular Weight: | 40,861 Da |
| NCBI Full Name: | HLA class I histocompatibility antigen, Cw-3 alpha chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class I, C |
| NCBI Official Symbol: | HLA-C |
| NCBI Official Synonym Symbols: | MHC; HLAC; HLC-C; D6S204; PSORS1; HLA-JY3 |
| NCBI Protein Information: | HLA class I histocompatibility antigen, Cw-1 alpha chain |
| UniProt Protein Name: | HLA class I histocompatibility antigen, Cw-3 alpha chain |
| UniProt Synonym Protein Names: | MHC class I antigen Cw*3 |
| Protein Family: | HLA class I histocompatibility antigen |
| UniProt Gene Name: | HLA-C |
| UniProt Entry Name: |
Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |
Applications: |
ELISA |
Applications: |
IHC |
Applications: |
IF |
Antibody Type: |
Polyclonal Antibody |
Conjugation: |
Unconjugated |
Related Products
HLA-B Antibody
Overview Product Name:HLA-B AntibodyProduct Code:PACO64761Size:50µLTarget Names:HLA-BSpecies Reactivity:HumanHos