Name: HLA-B Antibody (PACO33560)
SKU: PACO33560
Availability: OutOfStock

Antibody Name:	HLA-B Antibody (PACO33560)
Antibody SKU:	PACO33560
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human HLA-B protein (25-308AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: HLA-B antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa .
	Immunofluorescent analysis of HepG2 cells using PACO33560 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human spleen tissue using PACO33560 at dilution of 1:100.

Background:	Involved in the presentation of foreign antigens to the immune system
Synonyms:	HLA class I histocompatibility antigen, B-14 α chain (MHC class I antigen B*14), HLA-B, HLAB

UniProt Protein Function:	HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.Protein type: Immunoglobulin superfamily; Membrane protein, integralChromosomal Location of Human Ortholog: 6p21.3Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complexMolecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor bindingBiological Process: regulation of immune response; detection of bacterium; viral reproduction; regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response
UniProt Protein Details:
NCBI Summary:	HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96DW9
NCBI GenInfo Identifier:	8017397
NCBI Gene ID:	3106
NCBI Accession:	BC013187
UniProt Secondary Accession:	Q96DW9
UniProt Related Accession:	Q96DW9,P01889, P03989, P10319, P18463, P18464, P18465, P30460, P30461, P30462
Molecular Weight:	Calculated MW: 362aa, 40 kDaObserved MW: 40~45 kDa
NCBI Full Name:	HLA class I, partial
NCBI Synonym Full Names:	major histocompatibility complex, class I, B
NCBI Official Symbol:	HLA-B
NCBI Official Synonym Symbols:	AS; HLAB; SPDA1
NCBI Protein Information:	major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN00104; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; leukocyte antigen class I-B; lymphocyte antigen
UniProt Protein Name:	HLA class I
UniProt Synonym Protein Names:	HLA class I
Protein Family:	HLA class I histocompatibility antigen
UniProt Gene Name:	HLA-B
UniProt Entry Name:	Q9MYI4_HUMAN