Antibody Name:	HIPK1 Antibody (PACO57292)
Antibody SKU:	PACO57292
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Homeodomain-interacting protein kinase 1 protein (1007-1151AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO57292 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of Hela cells with PACO57292 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO57292 diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.

Synonyms:

Homeodomain-interacting protein kinase 1 (EC 2.7.11.1) (Nuclear body-associated kinase 2), HIPK1, KIAA0630 MYAK NBAK2

UniProt Protein Function:	HIPK1: homeodomain interacting protein kinase 1 is a CMGC kinase of the DYRK family. May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Expression elevated in many breast cancer cell lines. Null mice are resistant to carcinogens. Binds the p53 tumor suppressor. 4 alternatively spliced isoforms of the human protein have been described.Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamilyChromosomal Location of Human Ortholog: 1p13.2Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: eye development; positive regulation of angiogenesis
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86Z02
NCBI GenInfo Identifier:	34395641
NCBI Gene ID:	204851
NCBI Accession:	Q86Z02.1
UniProt Secondary Accession:	Q86Z02,O75125, Q5SQL2, Q5SQL4, Q5SQL5, Q8IYD7, Q8NDN5 Q8NEB6, Q8TBZ1, A6NJ34
UniProt Related Accession:	Q86Z02
Molecular Weight:	87,302 Da
NCBI Full Name:	Homeodomain-interacting protein kinase 1
NCBI Synonym Full Names:	homeodomain interacting protein kinase 1
NCBI Official Symbol:	HIPK1
NCBI Official Synonym Symbols:	Myak; Nbak2
NCBI Protein Information:	homeodomain-interacting protein kinase 1
UniProt Protein Name:	Homeodomain-interacting protein kinase 1
UniProt Synonym Protein Names:	Nuclear body-associated kinase 2
Protein Family:
UniProt Gene Name:	HIPK1
UniProt Entry Name:	HIPK1_HUMAN