HECW2 Antibody (PACO59525)
- SKU:
- PACO59525
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | HECW2 Antibody (PACO59525) |
| Antibody SKU: | PACO59525 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase HECW2 protein (495-641AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: HECW2 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 176, 136 kDa. Observed band size: 176 kDa. |
 | IHC image of PACO59525 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO59525 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Involved in the regulation of mitotic metaphase/anaphase transition. |
| Synonyms: | E3 ubiquitin-protein ligase HECW2 (EC 2.3.2.26) (HECT, C2 and WW domain-containing protein 2) (HECT-type E3 ubiquitin transferase HECW2) (NEDD4-like E3 ubiquitin-protein ligase 2), HECW2, KIAA1301 NEDL2 |
| UniProt Protein Function: | HECW2: E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ligase; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 2q32.3Cellular Component: cytoplasm; nucleusMolecular Function: ubiquitin-protein ligase activityBiological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9P2P5 |
| NCBI GenInfo Identifier: | 126215718 |
| NCBI Gene ID: | 57520 |
| NCBI Accession: | Q9P2P5.2 |
| UniProt Secondary Accession: | Q9P2P5,Q17RT5, Q68DF8, Q9NPS9, B8ZZB4 |
| UniProt Related Accession: | Q9P2P5 |
| Molecular Weight: | 135,905 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase HECW2 |
| NCBI Synonym Full Names: | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 |
| NCBI Official Symbol: | HECW2 |
| NCBI Official Synonym Symbols: | NEDL2 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase HECW2 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase HECW2 |
| UniProt Synonym Protein Names: | HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | HECW2 |
| UniProt Entry Name: | HECW2_HUMAN |
| Antibodies |
| HECW2 Antibody (PACO03044) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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