HECW1 Antibody (PACO63991)
- SKU:
- PACO63991
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | HECW1 Antibody (PACO63991) |
| Antibody SKU: | PACO63991 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase HECW1 protein (751-900AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Antigen Affinity purified & Affinity purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of A549 cells with PACO63991 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS. |
| Synonyms: | E3 ubiquitin-protein ligase HECW1 (EC 2.3.2.26) (HECT, C2 and WW domain-containing protein 1) (HECT-type E3 ubiquitin transferase HECW1) (NEDD4-like E3 ubiquitin-protein ligase 1) (hNEDL1), HECW1, KIAA0322 NEDL1 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q76N89 |
| NCBI GenInfo Identifier: | 559098413 |
| NCBI Gene ID: | 23072 |
| NCBI Accession: | NP_001273988.1 |
| UniProt Secondary Accession: | Q76N89,O15036, Q9HCC7, A7E2X0, A8MYS3, B4DH42 |
| UniProt Related Accession: | Q76N89 |
| Molecular Weight: | 179,554 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase HECW1 isoform b |
| NCBI Synonym Full Names: | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 |
| NCBI Official Symbol: | HECW1 |
| NCBI Official Synonym Symbols: | NEDL1 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase HECW1; HECT type E3 ubiquitin ligase; NEDD4-like ubiquitin-protein ligase 1 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase HECW1 |
| UniProt Synonym Protein Names: | HECT, C2 and WW domain-containing protein 1; NEDD4-like E3 ubiquitin-protein ligase 1; hNEDL1 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | HECW1 |
| UniProt Entry Name: | HECW1_HUMAN |
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