H2AFJ Antibody (PACO27293)
- SKU:
- PACO27293
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | H2AFJ Antibody (PACO27293) |
| Antibody SKU: | PACO27293 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Histone H2A.J protein (2-129AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: H2AFJ antibody at 2µg/ml Lane 1: Mouse brain tissue Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 15, 17 kDa Observed band size: 15 kDa . |
 | Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO27293 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human colon cancer using PACO27293 at dilution of 1:100. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | Histone H2A.J (H2a/j), H2AFJ |
| UniProt Protein Function: | H2AFJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 12p12.3Cellular Component: nuclear chromatinMolecular Function: DNA bindingBiological Process: chromatin silencing |
| UniProt Protein Details: | |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.[provided by RefSeq, Oct 2015] |
| UniProt Code: | Q9BTM1 |
| NCBI GenInfo Identifier: | 29553970 |
| NCBI Gene ID: | 55766 |
| NCBI Accession: | NP_808760.1 |
| UniProt Secondary Accession: | Q9BTM1,Q9NV63 |
| UniProt Related Accession: | Q9BTM1 |
| Molecular Weight: | 41.3kD |
| NCBI Full Name: | histone H2A.J |
| NCBI Synonym Full Names: | H2A histone family member J |
| NCBI Official Symbol: | H2AFJ |
| NCBI Official Synonym Symbols: | H2AJ |
| NCBI Protein Information: | histone H2A.J |
| UniProt Protein Name: | Histone H2A.J |
| UniProt Synonym Protein Names: | |
| Protein Family: | Histone |
| UniProt Gene Name: | H2AFJ |
| UniProt Entry Name: |
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