GST Monoclonal Antibody (MACO0651)

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SKU:
MACO0651
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
ELISA
Applications:
WB
Conjugation:
Unconjugated
Isotype:
IgG2a
Host Species:
Mouse

Description

Product Name:GST Monoclonal Antibody
Product Code:MACO0651
Size: 50µg
Target Names:GST
Species Reactivity:N/A
Host Species:Mouse
Antigen Species:N/A
Tested Applications:ELISA, WB; Recommended dilution:WB:1:500-1:5000
Isotype:IgG2a
Clone ID:N/A
Conjugate:Non-conjugated
Clonality:Monoclonal
Antigen:Recombinant GST Protein
Form:Liquid
Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:Protein G purified
Storage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Aliases:Glutathione S-Transferase, gst, GST tag
Uniprot ID:N/A
Background:Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Research Area:Others
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