GPR27 Antibody (PACO61229)
- SKU:
- PACO61229
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GPR27 Antibody (PACO61229) |
| Antibody SKU: | PACO61229 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Probable G-protein coupled receptor 27 protein (203-285AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61229 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO61229 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Orphan receptor. Possible candidate for amine-like G-protein coupled receptor. |
| Synonyms: | Probable G-protein coupled receptor 27 (Super conserved receptor expressed in brain 1), GPR27, SREB1 |
| UniProt Protein Function: | GPR27: Orphan receptor. Possible candidate for amine-like G- protein coupled receptor. Belongs to the G-protein coupled receptor 1 family.Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-passChromosomal Location of Human Ortholog: 3p21-p14Cellular Component: plasma membraneBiological Process: signal transduction |
| UniProt Protein Details: | |
| NCBI Summary: | GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010] |
| UniProt Code: | Q9NS67 |
| NCBI GenInfo Identifier: | 15214047 |
| NCBI Gene ID: | 2850 |
| NCBI Accession: | Q9NS67.1 |
| UniProt Secondary Accession: | Q9NS67 |
| UniProt Related Accession: | Q9NS67 |
| Molecular Weight: | 39,818 Da |
| NCBI Full Name: | Probable G-protein coupled receptor 27 |
| NCBI Synonym Full Names: | G protein-coupled receptor 27 |
| NCBI Official Symbol: | GPR27 |
| NCBI Official Synonym Symbols: | SREB1 |
| NCBI Protein Information: | probable G-protein coupled receptor 27 |
| UniProt Protein Name: | Probable G-protein coupled receptor 27 |
| UniProt Synonym Protein Names: | Super conserved receptor expressed in brain 1 |
| Protein Family: | Probable G-protein coupled receptor |
| UniProt Gene Name: | GPR27 |
| UniProt Entry Name: | GPR27_HUMAN |
| Antibodies |
| GPR27 Antibody (PACO03941) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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