GPR158 Antibody (PACO56402)
- SKU:
- PACO56402
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GPR158 Antibody (PACO56402) |
| Antibody SKU: | PACO56402 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Probable G-protein coupled receptor 158 protein (882-1003AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56402 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of U251 cells with PACO56402 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO56402 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Orphan receptor. |
| Synonyms: | Probable G-protein coupled receptor 158, GPR158, KIAA1136 |
| UniProt Protein Function: | GPR158: a member of the metabotropic glutamate family of G-protein coupled receptor 3 family.Protein type: GPCR, family 3; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integralChromosomal Location of Human Ortholog: 10p12.1Cellular Component: integral to membrane; plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; regulation of G-protein coupled receptor protein signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q5T848 |
| NCBI GenInfo Identifier: | 67461010 |
| NCBI Gene ID: | 57512 |
| NCBI Accession: | Q5T848.1 |
| UniProt Secondary Accession: | Q5T848,Q6QR81, Q9ULT3 |
| UniProt Related Accession: | Q5T848 |
| Molecular Weight: | 135,489 Da |
| NCBI Full Name: | Probable G-protein coupled receptor 158 |
| NCBI Synonym Full Names: | G protein-coupled receptor 158 |
| NCBI Official Symbol: | GPR158 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | probable G-protein coupled receptor 158 |
| UniProt Protein Name: | Probable G-protein coupled receptor 158 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Probable G-protein coupled receptor |
| UniProt Gene Name: | GPR158 |
| UniProt Entry Name: | GP158_HUMAN |
| Antibodies |
| GPR158 Antibody (PACO03043) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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