Name: GPR12 Antibody (PACO55526)
SKU: PACO55526
Availability: OutOfStock

Antibody Name:	GPR12 Antibody (PACO55526)
Antibody SKU:	PACO55526
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human G-protein coupled receptor 12 protein (1-48AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: K562 whole cell lysate. All lanes: GPR12 antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.
	Immunofluorescence staining of Hela cells with PACO55526 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO55526 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production.
Synonyms:	G-protein coupled receptor 12, GPR12

UniProt Protein Function:	GPR12: Promotes neurite outgrowth and blocks myelin inhibition in neurons. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integralChromosomal Location of Human Ortholog: 13q12Cellular Component: integral to plasma membraneMolecular Function: G-protein coupled receptor activity; phosphatidylcholine bindingBiological Process: cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P47775
NCBI GenInfo Identifier:	4885295
NCBI Gene ID:	2835
NCBI Accession:	NP_005279.1
UniProt Secondary Accession:	P47775,Q5T8P3
UniProt Related Accession:	P47775
Molecular Weight:	36,730 Da
NCBI Full Name:	G-protein coupled receptor 12
NCBI Synonym Full Names:	G protein-coupled receptor 12
NCBI Official Symbol:	GPR12
NCBI Official Synonym Symbols:	GPCR12; GPCR21; PPP1R84
NCBI Protein Information:	G-protein coupled receptor 12; protein phosphatase 1, regulatory subunit 84
UniProt Protein Name:	G-protein coupled receptor 12
UniProt Synonym Protein Names:
Protein Family:	G-protein coupled receptor
UniProt Gene Name:	GPR12
UniProt Entry Name:	GPR12_HUMAN