Glutathione Reductase Assay Kit (BA0087)

Glutathione Reductase Assay Kit - Information

Assay Genie's non-radioactive, colorimetric Glutathione Reductase Assay Kit is designed to accurately measure glutathione reductase (GR) activity in biological samples with a method that utilizes Ellman's method in which DTNB reacts with the GSH generated from the reduction of GSSG by glutathione reductase in a sample to form a yellow product (TNB^2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione reductase activity in the sample.

Applications

For quantitative determination of glutathione reductase activity.

Glutathione Reductase Assay Kit - Key Features

• Fast and sensitive. Linear detection range (20 sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Glutathione Reductase Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL GDH: 120 uL Substrate: 1 mL DTNB: 60 uL Cosubstrate: 1 mL Calibrator: 1.5 mL
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader
Method of Detection	OD412nm
Detection Limit	0.4 U/L
Samples	Plasma, serum, tissue and culture media etc
Species	All
Protocol Length	30 min
Size	100 tests
Storage	Store all components at -20°C upon receiving
Shelf Life	6 months

More Details

GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. Assay Genie' non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman's method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB^2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample