Name: Glutathione Peroxidase Assay Kit (BA0117)
Brand: Metabolism Assays
SKU: BA0117
Availability: OutOfStock

Glutathione Peroxidase Assay Kit (BA0117)

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SKU:

BA0117

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Biological Samples

Research Area:

Oxidative Stress

Research Area:

Reactive Oxygen Species

Research Area:

Plant & Environmental Stress

Research Area:

Enzyme Activity

Description
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Description

Glutathione Peroxidase Assay Kit

Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct and high-throughput assays for GPX activity find wide applications.

Assay Genie' improved glutathione peroxidase assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.

Applications

For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity.

Key Features

Sensitive and accurate. Use 10 uL sample. Linear detection range 40 to 800 U/L GPX activity.

Glutathione Peroxidase Assay Kit Information:

Kit Includes:	Assay Buffer: 25 mL GR Enzyme: 1 mL Glutathione: Dried NADPH: Dried Calibrator: Dried Peroxide Solution: 50 uL
Kit Requires:	Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, plate reader capable of reading optical density at 340 nm every minute, homogenizer etc.
Method of Detection:	OD340nm
Detection Limit:	40 U/L
Samples:	Biological
Species:	All
Protocol Length:	20 min
Size:	100 tests
Storage:	Store all components at -20°C.
Shelf Life:	6 months