Metabolism Assays
Glutamine Assay Kit (Colorimetric) (BA0113)
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- SKU:
- BA0113
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Animal Tissue, Red Blood Cells, Cultured Cells
- Research Area:
- Ferroptosis
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Amino Acid and Protein
- Research Area:
- Clinical Chemistry
Description
Glutamine Assay Kit - Information
Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, anabolic processes and is utilized for cellular energy and as a carbon source. It is used in treatment of injury, trauma, burns, and also as a supplement for muscle growth and post-surgery healing. Simple, direct and automation-ready procedures for measuring glutamine concentration are very desirable.
Assay Genie's glutamine assay kit is based on hydrolysis of glutamine to glutamate and colorimetric determination of the product. The intensity of the product color, measured at 565 nm, is proportional to the glutamine concentration in the sample.Applications
For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism.Key Features
- Sensitive and accurate. Use 20 uL sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Glutamine Assay Kit Information
| Kit Includes: | Assay Buffer: 15 mL NAD Solution: 1 mL Enzyme A: 120 mL MTT Solution: 2 x 1.5 mL Enzyme B: 220 mL Stop Reagent: 25 mL Standard: 400 mL 100 mM Glutamine |
| Kit Requires: | Pipeting (multi-channel) devices. Clear flat-bottom 96-well plates and plate reader. |
| Method of Detection: | OD565nm |
| Detection Limit: | 23 uM |
| Samples: | Serum, plasma, urine, cell, tissue etc |
| Species: | All |
| Protocol Length: | 40 min |
| Size: | 100 tests |
| Storage: | Store all reagents at -20°C |
| Shelf Life: | 6 months |
| Neto et al. | 2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identified | bioRxiv 2023 | View Citation |
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