Glutaminase (GLS) Activity Assay Kit
- SKU:
- MAES0230
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Animal, Plant Tissue
- Sensitivity:
- 0.003 U/L
- Range:
- 0.003-18.0 U/L
- Assay Time:
- 80min
Description
| Product Name: | Glutaminase (GLS) Activity Assay Kit |
| Product Code: | MAES0230 |
| Sample Type: | Serum (Plasma), Animal, Plant Tissue |
| Assay Time: | 80min |
| Assay Type: | Enzyme Activity |
| Synonyms: | GLS |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.003 U/L |
| Detection Range: | 0.003-18.0 U/L |
| Inter-assay CV: | 0.083 |
| Intra-assay CV: | 0.05 |
Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | PBS (0.01 mol/L, pH 7.4) |
| Other Instruments Required: | Test tube, Incubator, Centrifuge |
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