Description
Product Name: | Glutamate Dehydrogenase (GLDH) Activity Assay Kit |
Product Code: | MAES0231 |
Sample Type: | Serum (Plasma), Urine, Animal Tissue |
Assay Time: | 35 min |
Assay Type: | Enzyme Activity |
Synonyms: | GDH |
Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 0.54 U /L |
Detection Range: | 0.54-25.0 U/L |
Inter-assay CV: | 0.04 |
Intra-assay CV: | 0.03 |
GDH catalyzes the dehydrogenation of glutamate, meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of GDH can be calculated by measuring the change of absorbance value at 450 nm.
Storage: | 2-8°C |
Valid Period: | 12 months |
Other Reagents Required: | Double distilled water |
Other Instruments Required: | Incubator(37℃), centrifuge |