Description
| Antibody Name: | GFP Antibody (PACO13759) |
| Antibody SKU: | PACO13759 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:10000 |
| Species Reactivity: | Human |
| Immunogen: | Fusion protein of Aequorea Victoria GFP |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
![]() | Gel: 8%SDS-PAGE, Lysate: 1,5,10,20 μg, Lane: GFP transfected 293T cells, Primary antibody: PACO13759(GFP Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds. |
| Background: | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. |
| Synonyms: | Green fluorescent protein |
| UniProt Protein Function: | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P42212 |
| NCBI GenInfo Identifier: | 1169893 |
| NCBI Gene ID: | |
| NCBI Accession: | P42212.1 |
| UniProt Secondary Accession: | P42212,Q17104, Q27903, Q93125 |
| UniProt Related Accession: | P42212 |
| Molecular Weight: | |
| NCBI Full Name: | Green fluorescent protein |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Green fluorescent protein |
| UniProt Synonym Protein Names: | |
| Protein Family: | Green fluorescent protein |
| UniProt Gene Name: | GFP |
| UniProt Entry Name: | GFP_AEQVI |
