GenieFluor Violet 450 Anti-Mouse CD22 Antibody [Cy34.1] (AGEL3355)

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SKU:
AGEL3355
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Reactivity:
Mouse
Host Species:
Mouse
Isotype:
Mouse IgG1, k
Clone:
Cy34.1
Conjugation:
AFViolet 450

Description

Product Name:AFViolet 450 Anti-Mouse CD22 Antibody [Cy34.1]
Product Code:AGEL3355
Size:25ug, 100ug
Clone No:Cy34.1
Synonyms:B-cell receptor CD22, Cd22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, Lyb-8, Siglec2
Reactivity:Mouse
Host Species:Mouse
Applications:FCM
Isotype:Mouse IgG1, κ
Isotype Control:PE/TR Armenian Hamster IgG Isotype Control [PIP]
Conjugation:AFViolet 450
Applications:FCM
Recommended Dilution:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Storage:Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Fluorescent Label:GenieFluor Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).
Shipping:Biological ice pack at 4°C
Form:Liquid
Gene ID:12483
Swissprot:P35329
Background Information:The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.
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