GATB Antibody (PACO59836)
- SKU:
- PACO59836
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GATB Antibody (PACO59836) |
| Antibody SKU: | PACO59836 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate. All lanes: GATB antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO59836 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59836 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). |
| Synonyms: | Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial (Glu-AdT subunit B) (EC 6.3.5) (Cytochrome c oxidase assembly factor PET112 homolog), GATB, PET112 PET112L |
| UniProt Protein Function: | PET112L: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatB/GatE family. GatB subfamily.Protein type: Translation; Mitochondrial; Ligase; EC 6.3.5.-; RNA-bindingChromosomal Location of Human Ortholog: 4q31.3Cellular Component: mitochondrionMolecular Function: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; translation factor activity, nucleic acid bindingBiological Process: mitochondrial translation; translation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O75879 |
| NCBI GenInfo Identifier: | 7674164 |
| NCBI Gene ID: | 5188 |
| NCBI Accession: | O75879.1 |
| UniProt Secondary Accession: | O75879,Q4W5M8, Q53GP4, Q9P0S6, Q9Y2B8 |
| UniProt Related Accession: | O75879 |
| Molecular Weight: | 61,864 Da |
| NCBI Full Name: | Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial |
| NCBI Synonym Full Names: | glutamyl-tRNA amidotransferase subunit B |
| NCBI Official Symbol: | GATB |
| NCBI Official Synonym Symbols: | PET112; HSPC199; PET112L |
| NCBI Protein Information: | glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial |
| UniProt Protein Name: | Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial |
| UniProt Synonym Protein Names: | Cytochrome c oxidase assembly factor PET112 homolog |
| Protein Family: | PTS system galactitol-specific EIIB component |
| UniProt Gene Name: | GATB |
| UniProt Entry Name: | GATB_HUMAN |
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