Gallus Cytokine Array (10 targets) (SARB0014)

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SKU:
SARB0014
Product Type:
Protein Array
Analytes:
Caronte
Analytes:
IFN-gamma
Analytes:
IL-6
Analytes:
IL-10
Analytes:
IL-12 p40
Analytes:
IL-16
Analytes:
IL-21
Analytes:
Netrin-2
Analytes:
Pentraxin-3 (TSG-14)
Analytes:
RANTES (CCL5)
Reactivity:
Chicken
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0014
Size:2, 4, or 8
Species Detected:Gallus
Number of Targets Detected:10
Gene Symbols:CAR, CCL5, IFNG, IL10, IL12B, IL16, IL21, IL6, NTN2, NTN3|PTX3, TNFAIP5, TSG14
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Caronte
  • Gene Symbol: CAR
  • Gene ID: 395623
  • Accession #: Q9PUK2
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 396054
  • Accession #: P49708
IL-6
  • Gene Symbol: IL6
  • Gene ID: 395337
  • Accession #: Q90YI0
IL-10
  • Gene Symbol: IL10
  • Gene ID: 428264
  • Accession #: Q6A2H4
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 404671
  • Accession #: Q6X0K9
IL-16
  • Gene Symbol: IL16
  • Gene ID: N/A
  • Accession #: F1P243
IL-21
  • Gene Symbol: IL21
  • Gene ID: 554218
  • Accession #: Q58IU6
Netrin-2
  • Gene Symbol: NTN2 NTN3
  • Gene ID: 396387
  • Accession #: Q90923
Pentraxin-3 (TSG-14)
  • Gene Symbol: PTX3 TNFAIP5 TSG14
  • Gene ID: 5806
  • Accession #: P26022
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 417465
  • Accession #: Q8QG57
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Gallus (Chicken) Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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