β - galactosidase (β –GAL) Activity Assay Kit

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SKU:
MAES0215
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Cells, Animal And Plant Tissue
Sensitivity:
16.19 U/L
Range:
16.19-1200 U/L
Assay Time:
70 min

Description

Product Name:β - galactosidase (β –GAL) Activity Assay Kit
Product Code:MAES0215
Sample Type:Serum, Plasma, Cells, Animal And Plant Tissue
Assay Time:70 min
Assay Type:Enzyme Activity
Synonyms:β-GAL
Detection Instrument:Microplate reader (390-410 nm,optimum wavelength: 400 nm)
Detection Method:Colorimetric method
Sensitivity:16.19 U/L
Detection Range:16.19-1200 U/L
Inter-assay CV:0.06
Intra-assay CV:0.03

β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm.

Storage:Reagent 2: -20°C; Others: 2-8°C
Valid Period:12 months
Other Reagents Required:2 mmol/L hydrochloric acid
Other Instruments Required:Water bath, Incubator, Centrifuge, Microplate reader
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