β - galactosidase (β –GAL) Activity Assay Kit
- SKU:
- MAES0215
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Cells, Animal And Plant Tissue
- Sensitivity:
- 16.19 U/L
- Range:
- 16.19-1200 U/L
- Assay Time:
- 70 min
Description
| Product Name: | β - galactosidase (β –GAL) Activity Assay Kit |
| Product Code: | MAES0215 |
| Sample Type: | Serum, Plasma, Cells, Animal And Plant Tissue |
| Assay Time: | 70 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | β-GAL |
| Detection Instrument: | Microplate reader (390-410 nm,optimum wavelength: 400 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 16.19 U/L |
| Detection Range: | 16.19-1200 U/L |
| Inter-assay CV: | 0.06 |
| Intra-assay CV: | 0.03 |
β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm.
| Storage: | Reagent 2: -20°C; Others: 2-8°C |
| Valid Period: | 12 months |
| Other Reagents Required: | 2 mmol/L hydrochloric acid |
| Other Instruments Required: | Water bath, Incubator, Centrifuge, Microplate reader |
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