GAK Antibody (PACO54786)
- SKU:
- PACO54786
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GAK Antibody (PACO54786) |
| Antibody SKU: | PACO54786 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cyclin-G-associated kinase protein (939-1086AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO54786 at dilution of 1:100. |
 | Immunofluorescence staining of HepG2 cells with PACO54786 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. |
| Synonyms: | Cyclin-G-associated kinase (EC 2.7.11.1), GAK |
| UniProt Protein Function: | GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK familyChromosomal Location of Human Ortholog: 4p16Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; vesicleMolecular Function: protein bindingBiological Process: Golgi organization and biogenesis |
| UniProt Protein Details: | |
| NCBI Summary: | In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| UniProt Code: | O14976 |
| NCBI GenInfo Identifier: | 17375734 |
| NCBI Gene ID: | 2580 |
| NCBI Accession: | O14976.2 |
| UniProt Secondary Accession: | O14976,Q5U4P5, Q9BVY6 |
| UniProt Related Accession: | O14976 |
| Molecular Weight: | 134,464 Da |
| NCBI Full Name: | Cyclin-G-associated kinase |
| NCBI Synonym Full Names: | cyclin G associated kinase |
| NCBI Official Symbol: | GAK |
| NCBI Official Synonym Symbols: | DNAJ26; DNAJC26 |
| NCBI Protein Information: | cyclin-G-associated kinase |
| UniProt Protein Name: | Cyclin-G-associated kinase |
| UniProt Synonym Protein Names: | |
| Protein Family: | Cyclin-G-associated kinase |
| UniProt Gene Name: | GAK |
| UniProt Entry Name: | GAK_HUMAN |
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