FRYL Antibody (PACO61927)
- SKU:
- PACO61927
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | FRYL Antibody (PACO61927) |
| Antibody SKU: | PACO61927 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein furry homolog-like protein (42-739AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61927 diluted at 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of U251 cells with PACO61927 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator. |
| Synonyms: | Protein furry homolog-like (ALL1-fused gene from chromosome 4p12 protein), FRYL, AF4P12 KIAA0826 |
| UniProt Protein Function: | Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (). May function as a transcriptional activator. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O94915 |
| NCBI GenInfo Identifier: | 119874201 |
| NCBI Gene ID: | 285527 |
| NCBI Accession: | NP_055845.1 |
| UniProt Secondary Accession: | O94915,O95640, Q8WTZ5, Q9NT40 |
| UniProt Related Accession: | O94915 |
| Molecular Weight: | 45,597 Da |
| NCBI Full Name: | protein furry homolog-like |
| NCBI Synonym Full Names: | FRY like transcription coactivator |
| NCBI Official Symbol: | FRYL |
| NCBI Official Synonym Symbols: | MOR2; AF4p12; KIAA0826 |
| NCBI Protein Information: | protein furry homolog-like |
| UniProt Protein Name: | Protein furry homolog-like |
| UniProt Synonym Protein Names: | ALL1-fused gene from chromosome 4p12 protein |
| Protein Family: | Protein furry |
| UniProt Gene Name: | FRYL |
| UniProt Entry Name: |
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