FOXH1 Transcription Factor Activity Assay
- SKU:
- TFAB00110
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- FOXH1
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
Description
| Product Name: | FOXH1 Transcription Factor Activity Assay |
| Product Code: | TFAB00110 |
| Target: | FOXH1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie FOXH1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FOXH1: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:androgen receptor binding; bHLH transcription factor binding; DNA binding; protein binding; protein domain specific binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; SMAD binding; transcription factor activity Biological Process: anatomical structure morphogenesis; cell differentiation; negative regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway Disease: Holoprosencephaly 1 |
| NCBI Summary: | FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75593 |
| NCBI GenInfo Identifier: | 4503657 |
| NCBI Gene ID: | 8928 |
| NCBI Accession: | NP_003914.1 |
| UniProt Secondary Accession: | O75593,D3DWM4, |
| UniProt Related Accession: | O75593 |
| Molecular Weight: | 39,257 Da |
| NCBI Full Name: | forkhead box protein H1 |
| NCBI Synonym Full Names: | forkhead box H1 |
| NCBI Official Symbol: | FOXH1 |
| NCBI Official Synonym Symbols: | FAST1; FAST-1 |
| NCBI Protein Information: | forkhead box protein H1 |
| UniProt Protein Name: | Forkhead box protein H1 |
| UniProt Synonym Protein Names: | Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2 |
| Protein Family: | FAST kinase domain-containing protein |
| UniProt Gene Name: | FOXH1 |
