Description
Product Name: | Flag Tag Monoclonal Antibody |
Product Code: | MACO0660 |
Size: | 50µL |
Target Names: | Flag Tag |
Species Reactivity: | N/A |
Host Species: | Mouse |
Antigen Species: | N/A |
Tested Applications: | ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl |
Isotype: | IgG2a |
Clone ID: | 4G5C8 |
Conjugate: | Non-conjugated |
Clonality: | Monoclonal |
Antigen: | DYKDDDDK(Flag) synthetic peptide conjugate to KLH |
Form: | Liquid |
Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | Protein G purified |
Storage: | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Aliases: | DDK tag, DYKDDDDK, Flag, Flag Tag |
Uniprot ID: | N/A |
Background: | Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. |
Research Area: | Others |