FLAG PAT3G6AT Monoclonal Antibody [PAT3G6AT] (CPAB0693)

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SKU:
CPAB0693
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Mouse
Isotype:
IgG1
Clone:
PAT3G6AT
Applications:
ELISA
Applications:
FC

Description

system_update_alt Datasheet
Product Name:FLAG PAT3G6AT antibody
Product Sku:CPAB0693
Size:5μg
Host Species:Mouse
Immunogen:Anti-human FLAG mAb, is derived from hybridization of mouse F myeloma cells with spleen cells from BALB/c mice immunized with a Synthetic peptide (DYKDDDDKC)-KLH.
Clone:PAT3G6AT.
Reactivity:Other bodies
Applications:ELISA, Flow Cytometry
Purification Method:FLAG antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Isotype:IgG1
Background:A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
Synonyms:
Storage Buffer:For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.
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