FLAG Monoclonal Antibody [NYRFLAG] (CPAB0465)

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SKU:
CPAB0465
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Mouse
Isotype:
IgG1/kappa
Clone:
NYRFLAG

Description

system_update_alt Datasheet
Product Name:FLAG Antibody
Product Sku:CPAB0465
Size:500μg
Host Species:Mouse
Immunogen:Flag-conjugated proteins
Clone:NYRFLAG.
Reactivity:Other bodies
Applications:
Purification Method:Protein A & Ion exchange column.
Isotype:IgG1/kappa
Background:A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
Synonyms:
Storage Buffer:In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
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