FLAG Monoclonal Antibody, IgG2b [PFLAGSHG] (CPAB0486)

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SKU:
CPAB0486
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Mouse
Isotype:
IgG2b
Clone:
PFLAGSHG
Applications:
WB

Description

system_update_alt Datasheet
Product Name:FLAG Antibody, IgG2b
Product Sku:CPAB0486
Size:100μg
Host Species:Mouse
Immunogen:
Clone:PFLAGSHG.
Reactivity:Other bodies
Applications:Western Blot
Purification Method:
Isotype:IgG2b
Background:A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
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